Table 2.
Mass spectrometry protein identification of the HNE‐positive spots of AD I/II entorhinal cortex. HNE positive spots from all the AD cases were analyzed: spot #1 from AD case 5; spots #2, 3 and 4 from AD case 6; spot #5 from AD case 4, spots #6 and 7 from AD case 1; spot #8 from AD case 2; and spots #9 and 10 from AD case 3. Abbreviations: HNE = 4‐hydroxy‐2‐nonenal; AD = Alzheimer's disease; ATP = adenosine diphosphate.
| Spot | Protein | Theorical PI | Theorical MW | Sequence coverage | MOWSE score |
|---|---|---|---|---|---|
| 1 | gi|127798841ATP‐synthase, H + transporting mitochondrial F1 complex, α subunit | 9.07 | 59785 | 33% | 236* |
| 2 | gi|34782901 ATP‐synthase, H + transporting mitochondrial F1 complex, α subunit | 9.10 | 48879 | 21% | 90* |
| 3 | gi|34782901 ATP‐synthase, H + transporting mitochondrial F1 complex, α subunit | 9.10 | 48879 | 18% | 102* |
| 4 | gi|34782901 ATP‐synthase, H + transporting mitochondrial F1 complex, α subunit | 9.07 | 48879 | 18% | 162* |
| 5 | gi|4757810 ATP‐synthase, H + transporting mitochondrial F1 complex, α subunit | 9.16 | 59828 | 25% | 445§ |
| 6 | gi|4757810 ATP‐synthase, H + transporting mitochondrial F1 complex, α subunit | 9.16 | 59828 | 28% | 627§ |
| 7 | gi|127798841 ATP‐synthase, H + transporting mitochondrial F1 complex, α subunit | 9.07 | 59785 | 16% | 320§ |
| 8 | gi|4757810 ATP‐synthase, H + transporting mitochondrial F1 complex, α subunit | 9.14 | 59828 | 27% | 616§ |
| 9 | gi|127798841 ATP‐synthase, H + transporting mitochondrial F1 complex, α subunit | 9.07 | 59785 | 27% | 152* |
| 10 | gi|4757810 ATP‐synthase, H + transporting mitochondrial F1 complex, α subunit | 9.16 | 59828 | 42% | 980§ |
All the spots correspond to the mitochondrial ATP‐synthase α subunit.
Score values higher than 50 indicate identity or extensive homology (P < 0.05).
*
MALDI‐TOF/TOF used for the analysis.
§
ESI‐Q‐TOF used for the analysis.