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. Author manuscript; available in PMC: 2022 May 14.
Published in final edited form as: J Mol Biol. 2021 Mar 18;433(10):166948. doi: 10.1016/j.jmb.2021.166948

Figure 1 |. The NTD of TDP43 enhances LLPS of RBDsTDP43.

Figure 1 |

(a) The two constructs used in this figure, NTD-RBDsTDP43 (residues 1–273) and RBDsTDP43 (residues 100–273), each with a C-terminal His tag. NTD, N-terminal domain; RBD, RNA-binding domain. (b) Fluorescent confocal microscopy of each construct, with or without crowding agents. Each sample contains 50 μM total protein, consisting of 45 μM unlabeled protein and 5 μM protein labeled with fluorescein via maleimide conjugation. Scale bar is 5 μm. (c) Phase diagrams of RBDsTDP43 (left) and NTD-RBDsTDP43 (right) at varying salt and protein concentrations. LLPS determined by turbidity (Supplemental Figure S1a–b) and defined as absorbance > 0.3 AU.