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. 2008 Nov 24;20(1):119–132. doi: 10.1111/j.1750-3639.2008.00245.x

Figure 2.

Figure 2

RT‐PCR analysis of mRNA expression of p62 and LC3 in the NP0/0 cerebellum. mRNA levels of LC3B and p62 in both 3 month‐old (lane 3) and 6 month‐old mice (lane 4) are equivalent to the wild‐type (WT) (lane 2). The expression of b‐actin was analyzed as an internal control. Negative control samples which were not reverse transcribed (RT‐) are shown (lane 1) (A). Western blot analysis of the LC3B, p62 and Lamp1 proteins in the NP0/0 cerebellum. The 16‐kDa LC3B‐II protein, the 62 kDa p62 protein and the 110 kDa Lamp1 protein are increased in both 3–4 month‐old (lane 2) and 6–8 month‐old (lane 3) extracts of cerebellum from NP0/0 mice compared with adult wild‐type (lane 1) (B). As a control for equivalent protein loading, 42‐kDa actin was revealed.