Figure 2.
Downregulation of pro‐inflammatory and pro‐oxidative mediators due to enriched housing. (A) Western Blot analysis showed a decrease in cerebral prostaglandin E receptor 2 (PTGER2) amounts following enrichment (cropped images). (B) Densitometry analysis revealed a significant downregulation of PTGER2 by 53% (P = 0.0006). (C,D) In contrast, prostaglandin‐endoperoxide synthase 2 (PTGS2) Western Blot analysis identified unaltered cerebral protein amounts in both housing conditions (P = 0.361). (E,F) However, Western Blot analysis revealed diminished levels of activated Jun‐amino‐terminal kinases 2/3 (JNK2/3) (−49%, P = 0.047), whereas phosphorylated (p)‐JNK1 was not altered following enrichment (P = 0.245) (cropped images). Data are given as mean ± SEM, t‐test. EH = enriched housing; SH = standard housing. *P < 0.05, ***P < 0.001.