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. 2009 Jan 5;20(1):96–105. doi: 10.1111/j.1750-3639.2008.00249.x

Figure 1.

Figure 1

Repressor element‐1 silencing transcription factor/neuron‐restrictive silencer factor (REST/NRSF)‐target genes are expressed in lymphocytes. A. Schematic representation of the positions of the leukocyte immunoglobulin‐like receptor, subfamily B, member 4 (LILRB4) and immunoglobulin lambda‐like polypeptide 1 precursor (IGLL1) REST/NRSF target genes and repressor element‐1/neuron‐restrictive silencer element (RE1/NRSE) on chromosomes 19 and 22. The gray box identifies the gene, and the arrows indicate the transcriptional direction. The RE1/NRSE locus is indicated by the dark grey box. The distance of RE1/NRSE from the genes is calculated by taking the first gene nucleotide (the NC_000019 region from 59865936 to 59871660 for LILRB4 and the NC_000022 region (complement) from 22245312 to 22252495 for IGLL1) as 0. B. Qualitative polymerase chain reaction shows that LILRB4 and IGLL1 are expressed in lymphocytes, but they are not expressed in the cerebral cortex that expresses brain‐derived neurotrophic factor (BDNF) mRNA II, a neuronal isoform of BDNF under the control of REST/NRSF. The presence of three functional donor sites in human BDNF exon II leads to the production of the three BDNF isoforms, BDNF IIA, IIB and IIC. The glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) gene was amplified as a housekeeping gene (data are from one of three independent PCR experiments). Abbreviations: CTX = cortex; PBL = peripheral blood lymphocytes.