Table 3.
Synucleopathies.
| Reference | Disease/stage | Protein | Localization/function | Detection antibody | Degree of oxidation in relation to age‐matched controls | Enzymatic activity | Total protein levels | Comments |
|---|---|---|---|---|---|---|---|---|
| Choi et al (26) | PD | UCHL‐1 | UPS | Anti‐DNPH antibody | ↑ (10‐fold) | NA | NA | Identification of oxidation sites |
| Chung et al (30) | PD | Parkin | UPS | Anti‐S‐nitro‐sylated parkin antibody | ↑ | NA | = | Increase of S‐nitrosylated proteins measured by the Saville method parkin S‐nitrosylated by the biotin switch method |
| Yao et al (126) | PD | Parkin | UPS | Anti‐S‐nitroso‐parkin antibody | ↑ | NA | = | Detection of S‐nitrosylated proteins by the biotin switch method |
| Choi et al (28) | PD | SOD1 | Antioxidant response | Anti‐DNPH antibody | ↑ | NA | ↑ | Identification of oxidation sites |
| Dalfóet al (33) | iPD | β‐synuclein | Regulator of α‐synuclein aggregation | Anti‐MDAL antibody | ↑ | NA | NA | — |
| SOD2 | Antioxidant response | ↑ | NA | NA | — | |||
| Choi et al (29) | PD | DJ‐1 | Antioxidant response | Anti‐DNPH antibody | ↑ | NA | ↑ | Identification of oxidation sites |
| Keeney et al (56) | PD | Mitochondrial complex I | Electron transport chain | Anti‐DNPH antibody | ↑ | ↓ | = | Immunocapture to isolate the complete complex I in isolated mitochondria |
| Dalfó and Ferrer (32) | iPD | α‐synucleinnd | Synapsis | Anti‐MDAL antibody | ↑ | NA | NA | IP anti‐MDAL antibody |
| Gómez and Ferrer (48) | iPD PD DLB | Aldolase A | Glycolysis | Anti‐HNE antibody | ↑ | NA | NA | — |
| Enolase 1 | Glycolysis | ↑ | NA | NA | — | |||
| GADPH | Glycolysis | ↑ | NA | NA | — | |||
| Poon et al (95) | A30P α‐synuclein transgenic mice | LDH | Glycolysis | Anti‐DNPH antibody | ↑ | ↓ | NA | — |
| Enolase | Glycolysis | ↑ | ↓ | NA | — | |||
| Carbonic anhydrase | CO2 metabolism | ↑ | ↓ | NA | — | |||