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. 2021 Mar 26;296:100599. doi: 10.1016/j.jbc.2021.100599

Figure 2.

Figure 2

YAP mediates S1P-induced Notch3 expression and activation in PASMCs.A, PASMCs were treated with 1000-nM S1P for 48 h; the protein levels of Notch3 and NICD3 were examined using Western blotting; β-actin served as a loading control (n = 4 each group). B, PASMCs were transfected with YAP sequence-specific siRNA or nontargeting siRNA; the YAP protein level was determined using Western blotting; β-actin served as a loading control (n = 4 each group). C, PASMCs were previously transfected with YAP-specific or nontargeting siRNA for 24 h and then stimulated with 1000-nM S1P for 48 h; protein levels of Notch3 and NICD3 were detected using Western blotting; β-actin served as a loading control (n = 4 each group). ∗p < 0.05 versus control, #p < 0.05 versus control siRNA, &p < 0.05 versus control siRNA and S1P-cotreated cells. NICD3, intracellular domain of the Notch3; PASMCs, pulmonary artery smooth muscle cells; S1P, sphingosine-1-phosphate; YAP, yes-associated protein.