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. 2021 Mar 26;296:100599. doi: 10.1016/j.jbc.2021.100599

Figure 6.

Figure 6

β-TrCP promotes the ubiquitinated degradation of YAP in PASMCs.A, PASMCs were transfected with β-TrCP sequence-specific siRNA or nontargeting siRNA; β-TrCP protein level was examined using Western blotting; β-actin served as a loading control (n = 4 each group). B, PASMCs were transfected with β-TrCP sequence-specific siRNA or nontargeting siRNA or proteasome inhibitor MG-132; protein level of YAP was determined using Western blotting; β-actin served as a loading control (n = 4 each group). C, PASMCs were transfected with β-TrCP sequence-specific siRNA or nontargeting siRNA or proteasome inhibitor MG-132; ubiquitinated proteins were detected using Western blotting; β-actin served as a loading control (n = 4 each group). ∗p < 0.05 versus control siRNA, #p < 0.05 versus control. β-TrCP, β-transduction repeat-containing protein; PASMCs, pulmonary artery smooth muscle cells; YAP, yes-associated protein.