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. 2021 Mar 24;296:100589. doi: 10.1016/j.jbc.2021.100589

Figure 2.

Figure 2

Purification and primary biophysical studies of the pre-core promoter wt, G1738A, G1748A, and G1738/1748A mutant oligomers.A, the change in size-exclusion chromatography (SEC) elution profile suggests change in size of G4s. B, circular dichroism spectroscopy studies of the pre-core promoter oligomers. The presence of a negative peak at ∼242 nm and a positive peak at ∼263 nm indicates that the G4s are present at a parallel quadruplex in the solution, the respective values are tabulated in Table S1. The plots represent the average of the three independent runs for each.