Figure 4.

NGS analysis confirmed Insig1 ablation impacted metabolic and inflammatory pathways in WDSW and CCl₄models. (A) Prediction of upstream regulators significantly enriched and predicted (Z score) activated (orange) or inhibited (green) according to the IPA/URA in the Het and KO (vs WT) in the WDSW and CCl4 models (full list in Table S5). (B) Graphical representation in networks of differentially modulated genes (green, downregulated; red, upregulated) leading to the predicted activation (orange)/inhibition (blue) of the upstream regulators according to the IPA; data from the WDSW model. (C) Comparison of the genes differentially modulated in the treated Insig1 KO mice (vs WT), a subset of 175 genes were differentially modulated the WDSW and CCl₄ models. (p < 0.05; −0.378 ≤ log2 [fold change] ≥ 0.378; full list in Table S6). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)