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Barcode ZIKV contains high diversity at the engineered degenerate sites. (A) ZIKV was engineered with degenerative nucleotides in the third codon position of an 8-amino-acid stretch of NS2a, without altering the protein. (B to D) Barcode diversity is shown as individual barcodes by the percentage of the whole (B), the number of RNA molecules in the sequenced sample for each barcode identified (C), and the percentage of the total stock made up of each barcode (D).
