FIG 3.

Effects of nsP2 linker mutants on CHIKV production. (A) Effects of introduced mutations on the efficiency of viral rescue and titers of viral progeny. The experiment was repeated three times with similar results, and data from one reproducible experiment are shown. Capsid protein (top) and CHIKV nsP2 (bottom) expression in (B) BHK-21 and (C) U2OS cells transfected with plasmids containing icDNA of wt CHIKV or its mutants. (D) CHIKV nsP2 (top), CHIKV nsP3 (middle), and CHIKV nsP1 (bottom) expression in U2OS cells transfected with plasmids expressing the indicated replicases. On the Western blots, nsP1 to -3 and capsid proteins were detected using corresponding polyclonal antibodies; antibodies against actin were used to detect the loading control.