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. 2021 Feb 24;95(6):e01470-20. doi: 10.1128/JVI.01470-20

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FIG 4 — SDS-PAGE of the purified recombinant proteins. After purification using immobilized metal affinity chromatography (IMAC), the purified proteins were incubated with SUMO protease to remove the N-terminal His₆-SUMO tag before purification using heparin-Sepharose affinity chromatography. SDS-PAGE of (A) nsP2, (B) nsP2-K192A, (D) nsP2-delQ465, (E) nsP2-delSHQ, (F) nsP2-delSHQMT, (G) nsP2-insGSG, (H) nsP2-ins6GS, and (I) nsP2-ins10GS after heparin-Sepharose affinity chromatography. (C) Ten micromoles of purified recombinant proteins was separated using SDS-PAGE. Ovals, triangles, and squares indicate HS-nsP2-3E, HS-nsP2, and nsP2, respectively. HS, His₆-SUMO tag; E, EGFP tag.