TABLE 2.

ATP hydrolysis studies of the purified recombinant proteins in the presence or absence of RNA28

Protein	kcat (s−1)		Km for ATP (μM)		kcat/Km (s−1 μM−1)
	Apo	RNA28	Apo	RNA28	Apo	RNA28
nsP2	21.71 ± 0.40	35.37 ± 0.55	333.50 ± 21.75	331.90 ± 18.23	0.07 ± 0.02	0.11 ± 0.02
nsP2-delQ465	24.20 ± 0.51	46.07 ± 0.58	413.60 ± 29.19	257.70 ± 11.98	0.06 ± 0.01	0.18 ± 0.01
nsP2-delSHQ	29.00 ± 0.38	31.28 ± 0.49	269.30 ± 13.01	294.40 ± 16.48	0.11 ± 0.01	0.11 ± 0.01
nsP2-delSHQMT	17.57 ± 0.68	18.71 ± 0.84	894.00 ± 95.12	775.80 ± 99.60	0.02 ± 0.01	0.02 ± 0.01
nsP2-insGSG	32.10 ± 0.51	36.86 ± 0.60	269.70 ± 15.85	309.50 ± 18.00	0.12 ± 0.01	0.12 ± 0.01
nsP2-ins10GS	32.41 ± 0.46	34.88 ± 0.60	338.10 ± 16.96	339.20 ± 20.58	0.10 ± 0.00	0.10 ± 0.00
nsP2h	36.47 ± 0.48	37.70 ± 0.41	261.60 ± 12.61	278.30 ± 10.98	0.14 ± 0.02	0.14 ± 0.00
nsP2h + nsP2p	34.45 ± 0.57	37.22 ± 0.64	293.00 ± 17.45	337.30 ± 20.34	0.12 ± 0.06	0.11 ± 0.01
nsP2-K192A	0.67 ± 0.05		0.46 ± 0.66		0.24 ± 0.01
nsP2p	3.02 ± 0.56		1398.00 ± 628		0.00 ± 0.02