FIG 6.

Inhibition of IAV-induced SHP2 activation upregulates the expression of innate immunity-related genes. A549 cells were treated with 10 μM SHP099 (SHP) for 12 h, followed by infection with WSN (A and B), PR8 (C), or CA04 (D) (MOI = 1). A549 cells were transfected with siRNA corresponding to SHP2 (siSHP2) or scrambled control siRNA (siCtrl) at 120 nM for 24 h prior to WSN infection (MOI = 1) (F and G). After infection for 8 h, total RNA samples were harvested and subjected to qRT-PCR for assessing the levels of IFN and ISG mRNA. Following inhibition (E) and knockdown (H) of SHP2, IFN-β and IL-29 levels in the culture supernatants collected at 15 h after WSN infection were measured by ELISA. Data are means and SD. *, P < 0.05; **, P < 0.01.