FIG 3.
DNA (Southern) filter hybridization analysis of HBV DNA replication intermediates throughout postnatal liver development in HBV transgenic mice. (A) DNA (Southern) filter hybridization analysis of liver replication intermediates isolated from HBV transgenic mice of various postnatal developmental ages are shown. Noncontiguous lanes from a single analysis are presented. The probe used was HBVayw genomic DNA. The HBV transgene (Tg) was used as an internal control for the quantitation of the HBV replication intermediates. RC, HBV relaxed circular replication intermediates; SS, HBV single-stranded replication intermediates. (B) Quantitative analysis of DNA (Southern) filter hybridization of the HBV replication intermediates in the HBV transgenic mice. One-phase decay analysis was used to estimate the changes in HBV DNA replication intermediates throughout postnatal liver development in the HBV transgenic mice (P, plateau, maximum calculated number of HBV DNA replication intermediates per cell in adult mouse liver; t0.5, days required to reach half-maximal levels of HBV replication intermediates; GraphPad Prism 8.4).