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. 2021 Mar 26;296:100601. doi: 10.1016/j.jbc.2021.100601

Figure 1.

Figure 1

Downregulation of ADAR1 reduces the expression of P-gp in RPTECs.A, construction of ADAR1-knockdown (KD) RPTECs with stable expression of shRNA. The expression levels of ADAR1-p110 protein were normalized to that of β-ACTIN. Values are the mean with S.D. (n = 3). The value of ADAR1-p110 in mock-transduced RPTECs was set at 1.0. ∗∗p < 0.01; significant difference between the two groups (t4 = 6.988, p = 0.002; unpaired t-test, two-sided). B, the mRNA levels of solute carrier (SLC) transporters in mock-transduced and ADAR1-KD RPTECs. The mRNA levels were assessed by quantitative real-time RT-PCR analysis and their expression levels were normalized to that of 18S rRNA. Values are the mean with S.D. (n = 4). C, the mRNA levels of ABC transporters in mock-transduced and ADAR1-KD RPTECs. The mRNA levels were normalized to that of 18S rRNA. Values are the mean with S.D. (n = 3). ∗∗p < 0.01, ∗p < 0.05; significant difference between the two groups (t4 = 5.656, p = 0.005 for ABCB1; t4 = 4.175, p = 0.014 for ABCC2; unpaired t-test, two-sided). D, The protein levels of P-gp and MRP2 in mock-transduced and ADAR1-KD RPTECs. The protein levels were normalized to that of β-ACTIN. Values are the mean with S.D. (n = 3–4). ∗p < 0.05; significant difference between the two groups (t4 = 2.938, p = 0.043 for P-gp; unpaired t-test, two-sided).