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. 2021 Mar 27;48:101225. doi: 10.1016/j.molmet.2021.101225

Figure 1.

Figure 1

20β-DHB is a full agonist of the mineralocorticoid receptor. (A) In silico comparison of 20β-DHF and aldosterone binding on the human MR. (B) A dose–response curve of 20β-DHB and aldosterone in HEK293 cells transiently transfected with murine mineralocorticoid receptor. (C) Binding profile induced by aldosterone (red), cortisol (blue), or 20β-DHF (green) binding to mineralocorticoid receptor. Modulation index (MI) > 0 suggested ligand-favoured binding, while MI < 0 suggested ligand-disfavoured binding of a peptide compared to DMSO; ∗p < 0.05 and ∗∗p < 0.01. (D) Heat map depiction of details of ligand-induced binding of co-regulator peptides using MARCoNI. The colour of the bar represents the modulation index, that is, compound induced log-fold change of binding, red a positive fold change, and blue a negative fold change. (E-G) The correlation of co-regulator recruitment between 20β-DHF and aldosterone. (E) 20β-DHF and cortisol (F) and cortisol and aldosterone (G).