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. 2021 Mar 27;48:101225. doi: 10.1016/j.molmet.2021.101225

Figure 3.

Figure 3

Lean Cbr1 heterozygous mice had reduced 20β-DHB in plasma and adipose tissue (A) Cbr1 mRNA expression in subcutaneous adipose tissue of lean male and female mice (n = 5–7 mice/group). (B) Representative Western blotting of CBR1 in the subcutaneous adipose tissue from the lean male Cbr1+/+ and Cbr1+/− mice (n = 4 mice/group).(C)Quantification of CBR1 in the subcutaneous adipose tissue from the lean male and female Cbr1+/+ and Cbr1+/− mice. (D) CBR1 activity in the subcutaneous adipose tissue from the lean male and female Cbr1+/+ and Cbr1+/− mice (n = 4 mice/group). (E–F) 20β-DHB quantified by LC-MS/MS in the plasma and subcutaneous adipose tissue from the lean male and female Cbr1+/+ and Cbr1+/− mice (n = 4–6 mice/group). (G–H) Plasma corticosterone and aldosterone quantified by LC-MS/MS in the plasma from the lean male and female Cbr1+/+ and Cbr1+/− mice (n = 5–6 mice/group). Data are expressed as mean ± SEM. Statistical analysis was conducted with the Mann–Whitney U test. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.