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. 2021 Apr 20;11:638897. doi: 10.3389/fonc.2021.638897

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Copyright © 2021 Faumont, Taoui, Collares, Jais, Leroy, Prévaud, Jardin, Molina, Copie-Bergman, Petit, Gourin, Bordessoule, Troutaud, Baud and Feuillard

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Apoptosis of c-Rel overexpressed human B-cells. EREB2-5 cells were stably transfected with the doxycycline-regulatable pRT-1 vector coding for Luciferase (Luc), IκBα _S32,36A, IκBε, or c-Rel. After estradiol starvation for 24 h (EREB0), cells were treated with doxycycline (+ Dox) for 48 h. (A) Immunoblots detecting the expression of RelA, RelB, c-Rel, IκBα, IκBε, TRAF1, A20 as well as αTubulin as loading control. (B) Detection of cytoplasmic and nuclear c-Rel protein by immunoblot. SAM68 and αTubulin revelations are nuclear and cytoplasmic markers, respectively. (C) Percentages of Annexin-V-positive cells gated on NGFRt positive cells. Statistically significant difference (Mann–Whitney test) is indicated by *(p ≤ 0.05).