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. 2021 Apr 20;12:645085. doi: 10.3389/fendo.2021.645085

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Copyright © 2021 Müller, Robles, Zamora, Ebnet, Markl-Hahn, Martínez de la Escalera, Clapp, Bertsch and Triebel

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overview of the antibody development process. (A) A suitable, vasoinhibin-specific epitope at the loop 1 region (L1) was identified on the basis of a three-dimensional model of vasoinhibin as reported by Robles et al. (B) Antibodies obtained after immunization with two immunizing peptides comprising neighboring L1 regions were selected by their affinity to PRL, to the immunizing peptides, as well as to coated vasoinhibin antigen. (C) A sandwich ELISA consisting of two monoclonal, vasoinhibin-specific antibodies was set up.