Table 1.

ELISA performance characteristics.

Vasoinhibin standard curve in all sandwiches					0.1 – 20 µg/ml
Applied LOQ (lowest calibration standard)					100 ng
Intra-assay coefficient of variation of standard					12.5%
Inter-assay coefficient of variation of standard					14%
Cross-reactivity with PRL at 12 µg/ml at 5.5 µg/ml vasoinhibin standard (% deviation compared to vasoinhibin standard only)					C50-C10 = 3.3% C10-C50 = 7.3%
Sandwich	BLK [O.D.] (mean ± SD)	LOD [O.D.]	LOD - BLK [O.D.]	LOQ [O.D.]		LOQ – BLK [O.D.]
C1F-C50H	0.096 ± 0.011	0.129	0.033	0.206		0.110
C10F-C50H	0.768 ± 0.036	0.876	0.108	1.128		0.360
C50H-C10F	0.725 ± 0.071	0.938	0.213	1.435		0.710

BLK, blank; LOD, limit of detection; BLK mean plus the three-fold standard deviation of blank, LOQ, limit of quantitation, the applied limit of quantitation was the lowest calibration standard of 100 ng, the LOQ is the BLK-mean plus the 10-fold standard deviation of blank. LOD and LOQ are also shown as BLK-subtracted values for comparison with the standard curves.