Table 2.

Blank-subtracted O.D. values and the corresponding vasoinhibin (Vi) concentrations using monoclonal anti-vasoinhibin sandwich ELISA in a variety of human serum samples.

Sample	Serum ID	C10-C50, S1	C10-C50, S2	C1-C50, S1	C1-C50, S2	Vi-concentration	PRL concentration
		O.D., mean ± SD				µg/ml	ng/ml
1	3780-146-8	3.660 ± 0.115	3.711 ± 0.035	3.464 ± 0.155	3.545 ± 0.062	>STD/605.85	15.6
2	2866-146-22	0.073 ± 0.006	0.358 ± 0.008	-0.014 ± 0.001	0.014 ± 0.001	0.35/<LOD	10.1
3	3780-146-9	–	0.502 ± 0.016	0.550 ± 0.032	0.634 ± 0.025	2.54/25.48/26.48	7.8
4	3780-146-1	0.530 ± 0.020	–	0.045 ± 0.006	0.073 ± 0.009	3.24/<LOD	7.4
5	2869-146-5	0.327 ± 0.090	–	0.013 ± 0.001	0.037 ± 0.002	1.75/<LOD	5.1
6	3780-146-2	–	2.868 ± 0.078	0.516 ± 0.036	0.692 ± 0.049	41.63/24.08/29.09	23.9
7	3780-146-21	0.717 ± 0.012	–	0.080 ± 0.010	0.116 ± 0.005	4.55/1.77/2.25	10.0
8	2866-146-25	–	3.197 ± 0.035	0.711 ± 0.017	0.897 ± 0.036	64.61/43.27/38.86	4.4
9	3780-146-5	–	2.048 ± 0.013	0.334 ± 0.020	0.368 ± 0.015	11.46/12.88/17.64	4.8
10	2866-146-23	–	–	0.182 ± 0.003	0.242 ± 0.005	5.49/6.28	7.2
11	3780-146-3	–	–	0.686 ± 0.066	0.839 ± 0.025	36.83/39.0	–
12	2866-146-24	-0.013 ± 0.012	0.039 ± 0.016	-0.014 ± 0.001	0.007 ± 0.001	<LOQ/<LOD	–
13	2866-146-19	–	–	1.858 ± 0.099	2.340 ± 0.045	194.11/227.63	–
14	2869-146-3	0.060 ± 0.010	0.238 ± 0.033	-0.009 ± 0.003	0.014 ± 0.003	0.23/<LOD	–
15	3780-146-20	–	–	-0.008 ± 0.010	0.013 ± 0.001	<LOD	–

Four series (S) of measurements with different dilutions were done, evaluating sandwiches C10-C50 and C1-C50, twice each. O.D. values are not correlated with PRL concentrations (spearman correlation coefficient across all series r = 0.09).