FIGURE 3.

PCR of the Ds empty donor site (EDS), mCherry, and GFP sequences in T0 rice plants transformed with pBDL23 and pBDL22. Ten pBDL23- or pBDL22-transformed T0 rice plants (1–10) were tested. Ds excision in T0 plants was monitored by the EDS bands. pBDL10A and pBDL34 are the progenitor vectors of pBDL23 and pBDL22 before a Ds element was inserted into the SacI restriction site (see the section “Materials and Methods”), and were used as controls in EDS-PCR for the pBDL23- and pBDL22-transformed plants, respectively. GFP-EF and mCherry-ER are primers specific to the sequences adjacent to Ds element in pBDL23. GFP-EF and HPT-ER are primers specific to the sequences adjacent to Ds element in pBDL22.