TABLE 2.

Mutations in suppressors of ΔcvpA DOC sensitivity

Suppressor	Locus	Gene	Genome position	Nucleotide change	Amino acid changea	Mutationb	Functionc
1	RS00350	araC	75253	A 20 C	Asp 7 Ala	SNP	Arabinose operon transcriptional regulator
	RS02475	yajR	511698	G 1022 A	Gly 341 Asp	SNP	Putative transport protein
	RS13840	msbB	2635593	G 894 A	Trp 298 *	SNP	Lipid A biosynthesis acyltransferase
2	RS16885	nuoH	3209841	T 778A	Trp 260 Arg	SNP	NADH-quinone oxidoreductase subunit
	RS27795	lptG	5391979	G 187 A	Asp 63 Asn	SNP	LPS export ABC transporter permease
3	RS16885	nuoH	3209841	T 778 A	Trp 260 Arg	SNP	NADH-quinone oxidoreductase subunit
	RS21750	lptB	4174645	T 722 G	Leu 241 Arg	SNP	LPS export ABC transporter ATP-binding protein
4	RS13840	msbB	2635854	T 633 G	Asp 211 Glu	SNP	Lipid A biosynthesis acyltransferase
5	RS21750	lptB	4174548	A 625 C	Ile 209 Leu	SNP	LPS export ABC transporter ATP-binding protein
6	RS27795	lptG	5392372	A 580 C	Thr 194 Pro	SNP	LPS export ABC transporter permease
7	RS27795	lptG	5391946	G 154 C	Ala 52 Pro	SNP	LPS export ABC transporter permease
8	Intergenic	Upstream lptE	753329	C to T		SNP	LPS assembly lipoprotein
	RS02485	cyoD	513798	T 302 del	Asn 101 fs	DEL	Cytochrome bo terminal oxidase complex subunit IV
9	RS13840	msbB	2636269–2636276	CAGAGCGA 211–218 del	Ser 71 fs	DEL	Lipid A biosynthesis acyltransferase
10	RS16885	nuoH	3209841	T 778 A	Trp 260 Arg	SNP	NADH-quinone oxidoreductase subunit
	RS27795	lptG	5391979	G 187 A	Asp 63 Asn	SNP	LPS export ABC transporter permease
11	RS27790	lptF	5390961	C 269 A	Ala 90 Asp	SNP	LPS export ABC transporter permease
14	RS12645	dtpA	2407894	G 938 C	Gly 313 Ala	SNP	Proton-dependent di/tripeptide symporter
	RS02495	cyoB	516208	G 487 A	Gly 163 Ser	SNP	Cytochrome bo3 ubiquinol oxidase subunit
15	RS13840	msbB	2635801	C 686 A	Thr 229 Lys	SNP	Lipid A biosynthesis acyltransferase
	RS16885	nuoH	3209841	T 778 A	Trp 260 Arg	SNP	NADH-quinone oxidoreductase subunit
16	RS12645	dtpA	2407894	G 938 C	Gly 313 Ala	SNP	Di-/tripeptide permease
	RS02495	cyoB	516208	G 487 A	Gly 163 Ser	SNP	Cytochrome bo3 ubiquinol oxidase subunit

^aAsterisk, stop codon; fs, frameshift.

^bSNP, single-nucleotide polymorphism; DEL, deletion mutation.

^cLPS, lipopolysaccharide.