FIG 4.

Inclusion size, but not maturation, is dependent on functional DnaE. (A) HeLa cells were infected at an MOI of 0.2 with the parent (filled bars), the dnaE^ts mutant (gray bars), or dnaE^WT recombinant (white bars) strain and either were continuously incubated at 37°C or 40°C or were shifted between 37°C and 40°C at the times indicated. The cells were fixed at 34 hpi, labeled with an anti-LPS antibody, and imaged. Bars show the means for three experiments ± standard deviations. Two-way ANOVA and Tukey’s test were used to determine statistical significance. *, P < 0.05. (B) Cells infected with the dnaE^ts mutant were incubated at 37°C or 40°C for 24 h, fixed, and labeled with anti-MOMP and anti-IncA. Bars, 1 μm.