Figure 6.

Adipose tissue stromal cell (ATSC) extract alters the expression of signaling molecules regulating cell survival and proliferation within the lesioned spinal cord. A,B. Western blot analysis of spinal cord tissue harvested from uninjured, normal animals or at 1 week post spinal cord injury (SCI) from animals treated with Matrigel or Matrigel + ATSC extract showing expression of (A) neural proteins and acetylated histone H3 and H4 or (B) signaling molecules regulating cell survival and proliferation. Relative protein expression levels were quantified by densitometry. C. Semi‐quantitative RT‐PCR of inflammation related transcripts in spinal cord tissue harvested from uninjured, normal animals or at 1 week post SCI from animals treated with Matrigel or Matrigel + ATSC extract. PCR reactions were performed in triplicate for each tissue sample and then electrophoresed through a 1.5% agarose gel and photographed.