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. 2008 Mar 27;18(3):370–377. doi: 10.1111/j.1750-3639.2008.00130.x

Figure 2.

Figure 2

Tumor cells of high‐grade oligodendroglial tumors with poor prognosis are characterized by CSC and CD133 expression. A. Tumors with poor prognosis showed non‐adherent neurosphere‐like growth with few spheres of continuously increasing size within multiple small spheres showing limited growth. The growth pattern of primary glioblastomas (GBM) lacking an oligodendroglial component (R44) is shown for comparison (lower row). Representative photographs at DIV 7 and 21 are shown. B. The relative average sphere size of tumors with poor prognosis (R40, R52, R30, gray bars) as compared with GBM lacking an oligodendroglial component (R28 and R44, black bars) is given. Sphere size was determined weekly. C. Cancer stem cell (CSC) lines derived from tumors with poor prognosis were sorted for CD133 expression and plated with 10 cells/well. The number of spheres formed by CD133+ and CD133‐ cells after 14 days is given (clonogenic index: spheres per 100 cells plated). D. GBMs with oligodendroglial component were driven by CSC reconstituting a new tumor when injected into immunodeficient mice (n = 4) and were similarly tumorigenic as CD133+ and CD133‐ CSC isolated from primary GBM lacking an oligodendroglial component (2).