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Number of rats killed at various time points after the hypoxic exposure (in brackets) and their age‐matched controls for various methods (outside the brackets).
| Control (hypoxia) | Double Immunofluorescence | Real‐time RT‐PCR | Western blotting | Electron microscopy |
|---|---|---|---|---|
| 1 day (3 h) | 3 (3) | 5 (5) | 5 (5) | 3 (3) |
| 2 days (24 h) | 3 (3) | 5 (5) | 5 (5) | 3 (3) |
| 4 days (3 days) | 3 (3) | 5 (5) | 5 (5) | 3 (3) |
| 8 days (7 days) | 3 (3) | 5 (5) | 5 (5) | 3 (3) |
| 15 days (14 days) | 3 (3) | 5 (5) | 5 (5) | 3 (3) |
