Figure 10.

Utrn‐A and ‐B expression in different cellular components of the central nervous system. C2C12 cells, bEnd.3 cells, primary neurons and astrocytes cultures were used to study differential expression of Utrn‐A and ‐B proteins. A. C2C12 cells, primary mdx neurons and astrocytes showed greater Utrn‐A immunolabeling than Utrn‐B. C2C12 cells were colabeled with desmin, bEnd.3 cells, endothelial cell marker, CD31, neurons co‐labeled with the neuronal cell marker, NeuN, astrocytes colabeled with S100. B,D. Western blot analysis of lysates containing 50 µg of total protein probed with Utrn‐A and ‐B antibodies. Consistent with immunolabeling, Utrn‐A expression was higher in neurons and bEnd.3 cells compared with C2C12 cells and astrocytes. However, Utrn‐B protein expression was greater in bEnd.3 cells compared with other cell types. Expression of Utrn‐B in C2C12 cells was extremely low. Representative blots from a minimum of 5 experiments. Lower lanes show blots probed with tubulin antibodies. C,E. Histogram of densitometric quantification of Utrn‐A and Utrn‐B proteins in various cell types corrected by the corresponding tubulin bands, respectively. Compared with Utrn‐A and Utrn‐B protein levels in the C2C12 cells; statistical analysis was performed by one‐way analysis of variance (n = 6; *P < 0.05, **P < 0.001). Means are presented as ± SD. Scale bar, 100 µm.