Table 2.

Subjects included in the study. The abundance of CD8+ T‐cells in the TG was assessed by IHC, the IE by nested RT‐PCR, and the LAT‐positive neurons by ISH. Abbreviations: IE = immediate early; IHC = immunohistochemistry; ISH = in situ hybridization; LAT = latency‐associated transcripts; ND = not done; RT‐PCR = reverse transcription polymerase chain reaction; TG = trigeminal ganglia.

Case	CD8 IHC	ICP0 nRT‐PCR	ICP4 nRT‐PCR	LAT ISH
1*	41	Positive	Negative	26
2	59	Positive	Positive†	68
3	32	Negative	Negative	18
4*	34	Positive	Positive	39
5‡	41	Negative	Positive	10
6	45	Positive	ND	30
7‡	16	Positive	Positive	26
8	34	Positive	Negative	3
9	5	Positive	Negative	0§
10	100	Negative	Negative	46
11	38	Positive¶	Negative	20

^* These subjects were used for CDR3 spectratyping.

^† DNA contamination cannot be excluded.

^‡ These subjects were selected for single cell RT‐PCR.

^§ This subject tested positive only by LAT RT‐PCR.

^¶ This subject was tested by quantitative RT‐PCR for ICP0. A signal became discernible after 35 PCR cycles.

The infiltrating CD8+ T‐cells were counted at a magnification of ×400 in three fields of view that showed T‐cell clustering or accumulation. The mean number of CD8+ T‐cells is shown in column 2. For quantification of LAT, all neurons that were LAT‐positive in ISH were counted at a magnification of ×100. The number of LAT‐positive neurons is given in the last column.