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Single cell RT‐PCR on the TG of two subjects who showed a positive amplicon in the nested ICP0 or ICP4 RT‐PCR. Abbreviations: ND = not done; RT‐PCR = reverse transcription polymerase chain reaction; TG = trigeminal ganglia.
| Subjects | LAT(ISH)+/− neurons | LAT nRT‐PCR | ICP0 nRT‐PCR | ICP4 nRT‐PCR |
|---|---|---|---|---|
| 5 | + | 5/6 | 1/3 | 4/4 |
| − | 1/2 | ND | ND | |
| 7 | + | 2/2 | 3/5 | 3/5 |
| − | 0/1 | 0/1 | 1/1 |
5/6=indicates that 5 of 6 neuron pools were positive. One neuron pool consists of 3 to 5 laser cut neurons.
