Fig. 3. Short-term and long-term temperature exposures cause photophysiological differences among single cells of Symbiodinium CCMP2467 and CCMP421. (A) and (B) Single-cell maximum quantum yields (Fv/Fm) under incrementally increasing temperatures of short durations (15 min) for Symbiodinium CCMP2467 (A) and CCMP421 (B). Single cells were exposed to stepwise temperature treatments of +1 °C. Each temperature treatment is indicated by alternating red and white color bars. During the entire experiment Fv/Fm was assessed every 5 min and cells were otherwise kept in the dark. Right Y-axis and corresponding thin gray line: average temperatures as measured by sensors on the heating stage. Note the photophysiological traces of single-cells performing better (red circles, 2SD above) or worse (blue circles, 2SD below) than the population average (black triangles). (C) and (D) Single-cell maximum quantum yields (Fv/Fm) under incrementally increasing temperatures of long durations (6 h) for Symbiodinium CCMP2467 (C) and CCMP421 (D). Single cells were exposed to stepwise temperature treatments of +1 °C, each for a duration of 6 h. During long-term thermal exposures Fv/Fm was assessed every 15 min and cells were otherwise kept in the dark.
