Fig. 4. Sinusoidal temperature profiles cause progressively declining Fv/Fm in Symbiodinium CCMP2467. The top part shows measured temperatures during the application of a sinusoidal temperature profile that oscillates from 22 °C to 35 °C with a period of 30 min. At t = 0 h, the heaters were turned on and set to regulate at 22 °C. At 1 h 20 min, the oscillating profile was started. At 7 h 20 min a constant temperature of 22 °C was set. The bottom part shows the population average of single-cell maximum quantum yields (Fv/Fm) when exposed to sinusoidal temperature cycles. Error bars indicate ±2 SD. During the entire experiment Fv/Fm was assessed every 5 min and cells were otherwise kept in the dark.
