TABLE 2.
Primers used for Real-time PCR analysis.
| Genes# | Primers | Sequences (5′-3′) | Size, bp |
| SOD1 | Forward | GAGACCTGGGCAATGTGACT | 189 |
| Reverse | CCAAACGACTTCCAGCATTT | ||
| Nrf-2 | Forward | GAAAGCCCAGTCTTCATTGC | 190 |
| Reverse | TTGGAACCGTGCTAGTCTCA | ||
| GPX1 | Forward | AGCCCAACTTCATGCTCTTC | 159 |
| Reverse | CATTGCGACACACTGGAGAC | ||
| MyHC I | Forward | AAGGGCTTGAACGAGGAGTAGA | 114 |
| Reverse | TTATTCTGCTTCCTCCAAAGGG | ||
| MyHC IIa | Forward | GCTGAGCGAGCTGAAATCC | 136 |
| Reverse | ACTGAGACACCAGAGCTTCT | ||
| MyHC IIb | Forward | CACTTTAAGTAGTTGTCTGCCTTGAG | 83 |
| Reverse | GGCAGCAGGGCACTAGATGT | ||
| MyHC IIx | Forward | AGCTTCAAGTTCTGCCCCACT | 76 |
| Reverse | GGCTGCGGGTTATTGATGG | ||
| MyoD | Forward | CAACAGCGGACGACTTCTATG | 383 |
| Reverse | GCGCAAGATTTCCACCTT | ||
| MyoG | Forward | GCAGGGTGCTCCTCTTCA | 230 |
| Reverse | AGGCTACGAGCGGACTGA | ||
| β-actin | Forward | TGCGGGACATCAAGGAGAAG | 216 |
| Reverse | AGTTGAAGGTGGTCTCGTGG |
#SOD1, superoxide dismutase 1; Nrf-2, nuclear factor E2-related factor-2; GPX1, glutathione peroxidase 1; MyHC, myosin heavy chain; MyoD, myogenic differentiation factor 1; MyoG, myogenin; β-actin, housekeeping gene.