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Active-site labeling of PBPs and LDTs with β-lactam probes. (A) In PBPs, an active-site serine binds to a reactive β-lactam probe, as shown for Bocillin-FL. (B) In contrast, the LDT’s active-site cysteine binds to carbapenems (e.g., meropenem-Cy5). Insets: Active-site covalently bound to the β-lactam probe.
