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Structures of (A) β-lactam antibiotics and (B) activity-based probes used in the current work. The Cy5-conjugated derivatives were obtained by reacting the alkyne probe with sulfo-cyanine5 (Cy5) azide to produce Azt-Cy5, CephX-Cy5, and Mero-Cy5. The red star denotes the Cy5 fluorophore, and the β-lactam ring is highlighted in red.
