Table 2.
Minimum inhibitory concentrations of PPNA, PNA or peptide tested against wild-type (wt) Salmonella enterica serovar Typhimurium strain SL1344 (∼106 cfu/ml). Conjugates showing an inhibitory effect in the herein performed growth kinetics are highlighted in cyan. Respective growth curves for the indicated constructs are shown in Figure 2
| Name | Peptide sequence | PNA sequence | MIC in μM | MIC in μg/ml |
|---|---|---|---|---|
| KFF-acpP | KFFKFFKFFK | ctcatactct | 5 | 20 |
| KFF-acpP-scr | KFFKFFKFFK | tcactatctc | >10 | |
| KFF | KFFKFFKFFK | - | >10 | |
| RXR-acpP | RXRRXRRXRRXRXB | ctcatactct | 5 | 22 |
| RXR-acpP-scr | RXRRXRRXRRXRXB | tcactatctc | >10 | |
| RXR | RXRRXRRXRRXRX | - | >10 | |
| Tat-acpP | GRKKKRRQRRRYK | ctcatactct | 10 | 44 |
| Tat-acpP-scr | GRKKKRRQRRRYK | tcactatctc | >40 | |
| Tat | GRKKKRRQRRRYK | - | >40 | |
| acpP | - | ctcatactct | >40 |
X = 6-aminohexanoic acid
B = β-alanine
Peptide and PNA sequences are represented in N to C termini orientation.