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. 2021 Apr 13;49(8):4421–4440. doi: 10.1093/nar/gkab209

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — USP7 deubiquitinating H2BK120ub1 regulates the trimethylation of H3K27. (A) A375 cells transfected with USP7 siRNA were fixed and immunostained with the indicated antibodies followed by confocal microscopy analysis. The white arrows indicate cells with USP7 depletion to different extents; scale bar: 10 μm. (B) A375, HaCaT and PIG1 cells were co-transfected with Control siRNA, EZH2 siRNA, Vector or FLAG-EZH2 and different amounts of USP7 siRNA as indicated. Cellular extracts were collected and analyzed by western blotting. (C) A375 cells stably expressing FLAG-EZH2 transfected with USP7 siRNA, then fixed, and immunostained with the indicated antibodies followed by confocal microscopy analysis. The white arrows indicate cells with USP7 depletion to different extents; scale bar: 10 μm. (D) A375 cells were co-transfected with Control siRNA or different sets of USP7 siRNAs and different amounts of FLAG-EZH2 as indicated. Cellular extracts were collected and analyzed by western blotting.