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. 2021 Apr 6;49(8):4441–4455. doi: 10.1093/nar/gkab210

Figure 4.

Figure 4.

H3K27me3 and BAHD1 co-repress Polycomb target genes. (A) H3K27me3 and total H3 immunoblots using HEK293 cells post-treatment with 5 uM of DMSO or UNC1999 for three days. (B) Heatmap showing the relative expression of DEGs in HEK293 cells treated with 5 uM of UNC1999 (right) relative to DMSO (left) for three days, as revealed by RNA-seq (n = 3 biological replicates per group). (C) RT-qPCR of BAHD1 and the indicated BAHD1-repressed target gene in HEK293 cells post-treatment with 5 uM of UNC1999 versus DMSO for 3 days (n = 3 biologically independent samples). Data are presented as mean ± SD after normalization to GAPDH and then to mock-treated. n.s., not significant; ** P < 0.01; *** P < 0.001. (D) Venn diagram showing significant overlap between the DEGs upregulated due to expression of the W667G-mutated BAHD1 relative to WT (left) and those upregulated due to UNC1999 treatment relative to DMSO (right) in HEK293 cells. (E) Immunoblotting for f-BAHD1 (top) using the total extract (left) or the chromatin-bound and soluble fractions (right) of the HEK293 cells post-treatment of UNC1999, relative to DMSO. GAPDH and H3 served as the control of cell fractionation.