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. 2021 Apr 9;49(8):4668–4688. doi: 10.1093/nar/gkab223

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — The MMTV packaging signal RNA binds to Pr77^Gag. (A) Scheme of the 712 nts long in vitro transcribed unspliced gRNA (SA35), spliced env (AK29), and spliced sag (AK30) RNAs used for band-shift competition assays. (B) Representative gel of a band-shift assay using radiolabeled MMTV gRNA and increasing concentrations of Pr77^Gag. (C) Saturation plot fit to Hill equation based on the quantification of bands obtained in the band-shift assay. Best fit was obtained with plateau = 105 ± 6%, Hill coefficient = 3 ± 0.4 and K_d = 480 ± 26 nM (mean ± SD); R² = 0.99.