Figure 5.

Differential binding abilities of ssPurines and its mutant RNAs to MMTV Pr77^Gag. (A) Nature of mutations introduced in ssPurines that were used in band-shift competition assays. The red and underlined nucleotides represent substitutions introduced. (B–D) Data generated from quantification of gels analyzing the binding specificity of the listed ssPurine-mutant RNAs to full-length MMTV Pr77^Gag analyzed by band-shift competition assays. A radiolabeled 712 nts long unspliced WT gRNA and 800 nM MMTV Pr77^Gag were incubated with increasing concentrations of unlabeled competitor mutant RNAs. WT, wild type.