Figure 1.

Induction of inflammation in HEK-TLR4 cells. HEK-TLR4 cell cultures were treated with or without 100 ng/ml LPS to induce inflammation as described (see Methods). The Control cell cultures (left panel) were subsequently maintained in the dark in the incubator for 48 hours without further intervention. The Infra-Red treated cells (which included both LPS induced and non-induced cultures) were exposed to 720 nm Infrared light pulses for 10 minutes at 6 W/m2, at 12-hour intervals over a two day growth period(right panel). The inflammatory response (OD620/OD750) was quantified as the absorbance of the QUANTI-Blue SEAP substrate at 620 nm. normalized to the total protein concentration(absorbance at 750 nm)(see Methods). Infrared light was successful in reducing inflammation in LPS – stimulated (Grey Bars) but not unstimulated control cells (White Bars). Data are shown as the mean ± SE taken from five individual wells of one representative biological experiment