Table 2.
Analytical recovery of tetranor-PGDM in artificial urine samples with different tetranor-PGDM concentrations.
| Concentration (ng/mL) | Mean recoveries (%) | |||
|---|---|---|---|---|
| Sample | Spiked | Theoretical | Measured | |
| AU-1 (n = 3) | 0 | — | 0.85 | — |
| 0.86 | 1.32 | 1.49 | 113.5 | |
| 1.87 | 2.10 | 2.09 | 100.0 | |
| 3.91 | 3.67 | 3.81 | 103.8 | |
| 9.12 | 7.68 | 7.04 | 93.0 | |
| AU-2 (n = 3) | 0 | — | 5.95 | — |
| 0.86 | 5.25 | 5.53 | 105.6 | |
| 1.87 | 6.03 | 6.53 | 108.4 | |
| 3.91 | 7.60 | 6.99 | 91.9 | |
| 9.12 | 11.62 | 9.53 | 82.3 | |
Concentration of tetranor-PGDM was 0.85 (AU-1) and 5.95 (AU-2). The theoretical concentrations were corrected using the recovery value from the SPE procedure (77.1% by LC-MS/MS).