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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Free Radic Biol Med. 2021 Mar 17;167:94–108. doi: 10.1016/j.freeradbiomed.2021.02.010

Figure 4.

Figure 4.

Ex vivo mouse lens epithelium is susceptible to RSL3 induced ferroptosis. (A) Mouse lens capsular explants were treated with various concentration of RSL3 for 24hr, and cell death/ferroptosis was probed by propidium iodide. The same volume of DMSO treatment served as a control. Hoechst 33342 was used for the cell nucleus stain. (B) Fer-1 but not ZAVD-FMK blocked RSL3 mediated mouse lens epithelium ferroptosis. Mouse lens capsule explants were treated with 1μM RSL3 for 24hr w/ or w/o the presence of 10μM ZVAD-FMK and 0.1μM Fer-1. The cell death was determined by propidium iodide stain and the cell nucleus was visualized by Hoechst 33342 stain. Each assay was repeated three times and at least 10 images were collected for each stain and the representative images were shown.