Fig. 4.

Glutamine‐free diet impairs Notch1‐driven leukemia progression in vivo. (A) Schematic representation of the strategy followed for in vivo experiments. (B) Evolution of body weight of mice fed with a glutamine‐free diet for the indicated time. (C) Concentration of glutamine in the blood of mice implanted with either EV or NICD H33HJ‐JA1 cells fed under complete (+Q) or glutamine‐free (−Q) diet at the end of the treatment, as indicated. (D‐F) Representative luminescence images (D) and luminescence quantification (E‐F) of mice implanted with either EV or NICD cells fed under complete (+Q) or glutamine‐free (−Q) diet at the end of the treatment, as indicated. (G) Infiltration of leukemic human (CD3+) cells in the bone marrow of mice implanted with either EV or NICD cells, fed either in the presence or the absence of glutamine (Q). Graphs show mean values ± SEM (20 mice/group, *P < 0.05). Two‐way ANOVA followed by Bonferroni’s comparison as a post hoc test was used to evaluate the statistical difference between more than two groups.