Skip to main content
. 2021 Apr 10;15(5):1432–1449. doi: 10.1002/1878-0261.12928

Fig. 2.

Fig. 2

Mitochondrial STAT3 regulates expression of GSH biosynthetic genes and antioxidant genes. (A) Basal mRNA levels of GSH biosynthesis pathway genes (GCLC, GCLM, NQO1, and HMOX1) in MDA‐MB‐231 STAT3 WT and KO cells. (B) mRNA levels of antioxidant genes in MDA‐MB‐231 STAT3 KO cells after reconstitution with MTS‐STAT3. (C) mRNA levels of antioxidant genes in MDA‐MB‐231 STAT3 WT and KO cells after treatment with 50 µm rotenone or 250 µm Trolox for 8 h. (D) mRNA levels of antioxidant genes in MDA‐MB‐231 cells after reconstitution with NDI1 or treatment with 10 mm nicotinamide (NAM) for 16 h. (E) NAD+/NADH ratio measured in STAT3 WT, KO, KO + NDI1, or KO cells treated with 10 mm NAM for 48 h. Student’s t‐test: ***P < 0.001, **P < 0.01, *P < 0.05, ns = not significant, n = 3. Error bars represent ± SD.