Table 2.

Percentage increase/decrease in total cfDNA levels over each incubation period vs the percentage increase/decrease in the total number of live cells over the corresponding periods.

Human bone osteosarcoma (143B) cells
Time-segment	Change in total live cells (%)	Change in total cfDNA (%)
	Count and viability assay	qPCR	Pearson's r	p-value	Qubit	Pearson's r	p-value
4 to 8 h	33.18	85.52	0.11	0.84	76.70	0.2509	0.63
8 to 12 h	−0.65	37.79			37.85
12 to 16 h	−23.01	−5.28			−9.28
16 to 20 h	51.09	15.41			20.76
20 to 24 h	43.61	19.51			24.61
24 to 28 h	57.89	4.16			11.75

Primary dermal fibroblasts (PCS201010)
Time-segment	Change in total live cells (%)	Change in total cfDNA (%)
	Count and viability assay	qPCR	Pearson's r	p-value	Qubit	Pearson's r	p-value
4 to 8 h	7.08	87.36	−0.31	0.55	19.26	0.51	0.30
8 to 12 h	−15.57	−2.24			−8.57
12 to 24 h	79.11	36.97			31.24
24 to 36 h	58.49	−9.96			−3.85
36 to 56 h	76.02	7.67			18.20
56 to 80 h	82.80	−8.82			11.68

Human dermal microvascular endothelial cells (HMEC-1)
Time-segment	Change in total live cells (%)	Change in total cfDNA (%)
	Count and viability assay	qPCR	Pearson's r	p-value	Qubit	Pearson's r	p-value
4 to 8 h	−14.02	48.74	0.08	0.88	29.66	0.13	0.80
8 to 12 h	−2.23	4.98			12.04
12 to 24 h	14.20	90.66			77.59
24 to 36 h	24.61	39.23			39.86
36 to 56 h	65.80	28.36			24.61
56 to 80 h	44.76	72.24			57.28