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. 2021 May 4;12(5):442. doi: 10.1038/s41419-021-03622-x

Fig. 5. Liver-specific overexpression of FTO suppresses the IRI-elevated expression of Drp1 in hepatocytes upon IRI in vivo and in vitro.

Fig. 5

A Me-RIP qRT-PCR analysis was performed to evaluate the methylated Drp1 RNA level in the hepatocytes and hepatic tissues infected with control AAV8-TBG-null and AAV8-TBG-FTO during IRI. B qRT-PCR was adopted to analyze the expression of Drp1 at the mRNA level in the hepatocytes and hepatic tissues infected with control AAV8-TBG-null and AAV8-TBG-FTO during IRI. C Western blotting analysis was used to verify the expressions of total and activated Drp1 (pSer616) at the protein level in the hepatocytes and hepatic tissues infected with control AAV8-TBG-null and AAV8-TBG-FTO during IRI. N = 3; *P < 0.05 and **P < 0.01 vs. AAV8-TBG-NC group.