FIGURE 2.

Deposition characteristics at different concentrations, settling time, and multiple nebulization steps. Sodium fluorescein solution is deposited as described in the section “Materials and Methods.” Either 20 μl (A) or 200 μl (B) was used for nebulization. From left to right, 10-fold increasing concentrations were nebulized, the efficiency of different settling times after the end of nebulization processes was assessed, and respective doses nebulized one or more times into one well at 30-s settling time were analyzed, as indicated. Error bars show standard deviation. One-way ANOVA, Tukey’s multiple comparisons test, ns p > 0.12; ^∗p < 0.033; ^∗∗p < 0.002; ^∗∗∗p < 0.001. N = 9 of three experiments.